Publications

Kanfra X., Wrede A., Mahnkopp-Dirks F., Winkelmann T., Heuer H. (2022). Networks of free-living nematodes and co-extracted fungi, associated with symptoms of apple replant disease. Applied Soil Ecology, 172(), 104368. doi:10.1016/j.apsoil.2021.104368

Apple replant disease affects tree nurseries and apple production globally. After repeated planting in the same soil, apple roots show accumulation of phytoalexins, stunting, and blackening. Recently, we showed that nematodes extracted from replanted soil and co-extracted microbes triggered these sy…

Pielhop T., Popp C., Knierim D., Margaria P., Maiß E. (2022). Three new mycoviruses identified in the apple replant disease (ARD)-associated fungus Rugonectria rugulosa. Virus Genes, 58(5), 423-435. doi:10.1007/s11262-022-01924-6

AbstractIn this study, three new mycoviruses were identified co-infecting the apple replant disease (ARD)-associated root endophyte Rugonectria rugulosa. After dsRNA extraction, six viral fragments were visualized. Four fragments belong to a quadrivirus, which has a genome size of 17,166 bp. Each of…

Mahnkopp-Dirks, F., Winkelmann, T. (2022). Welche Rolle spielen Bakterien der Gattung Streptomyces in der Nachbaukrankheit bei Apfel?. Versuchsberichte Baumschule und Obstbau, Jahrgang 2021(), p. 26 - 31.

Benning S., Brugnone N., Siani R., Kublik S., Schloter M., Rad V. (2021). Complete Genome Sequences of Two Rhodococcus sp. Strains with Large and Linear Chromosomes, Isolated from Apple Rhizosphere. Microbiology Resource Announcements, 10(24), . doi:10.1128/mra.00159-21

Members of the genus Rhodococcus are usually able to catalyze a number of processes, which are of great interest for ecosystem performance as well as biotechnology. Here, we report the complete genome sequences of two Rhodococcus strains that were isolated from rhizosphere soil from an apple orchard…

Armin R., Zühlke S., Mahnkopp-Dirks F., Winkelmann T., Kusari S. (2021). Evaluation of Apple Root-Associated Endophytic Streptomyces pulveraceus Strain ES16 by an OSMAC-Assisted Metabolomics Approach. Frontiers in Sustainable Food Systems, 5(), . doi:10.3389/fsufs.2021.643225

The One Strain Many Compounds approach (OSMAC) is a powerful and comprehensive method that enables the chemo-diversity evaluation of microorganisms. This is achieved by variations of physicochemical cultivation parameters and by providing biotic and abiotic triggers to mimic microorganisms' natural …

Reim S., Cestaro A., Siewert C., Wöhner T., Mahnkopp-Dirks F., Winkelmann T., Hanke M., Flachowsky H. (2021). Evaluation of tolerance to apple replant disease (ARD) inMalusgermplasm. Acta Horticulturae, (1307), 327-334. doi:10.17660/actahortic.2021.1307.50

Apple replant disease (ARD), also described as soil decline, is known for centuries; however, up to now the ultimate causes are unknown. After replanting plants of the same species, the plants are characterized by a poor vegetative development, stunted growth and reduced yield in terms of quantity a…

Busnena B., Beuerle T., Mahnkopp-Dirks F., Winkelmann T., Beerhues L., Liu B. (2021). Formation and exudation of biphenyl and dibenzofuran phytoalexins by roots of the apple rootstock M26 grown in apple replant disease soil. Phytochemistry, 192(), 112972. doi:10.1016/j.phytochem.2021.112972

Apple replant disease (ARD) is a severe soil-borne disease frequently observed in apple tree nurseries and orchards worldwide. One of the responses of apple trees to ARD is the formation of biphenyl and dibenzofuran phytoalexins in their roots. However, there is no information on whether or not thes…

Rohr, A. D., Winkelmann, T. (2021). Frühe Indikatoren für die Nachbaukrankheit bei Apfel. Versuchsberichte Baumschule und Obstbau, (), p. 21 - 25.

Rohr A., Schimmel J., Liu B., Beerhues L., Guggenberger G., Winkelmann T. (2021). Identification and validation of early genetic biomarkers for apple replant disease. PLOS ONE, 15(9), e0238876. doi:10.1371/journal.pone.0238876

Apple replant disease (ARD) is a serious threat to producers of apple trees and fruits worldwide. The ARD etiology is not unraveled and managing options are either economically not applicable or environmentally harmful. Thus, interest is given in biomarkers that allow to indicate ARD situations at e…

Grunewaldt-Stöcker, G., Popp, C., Wamhoff, D., Maiss, E. and Winkelmann, T. (2021). Microscopic evidence of Nectriaceae and other microbes in infected fine root tissue of replant diseased apple plants. European Journal of Horticultural Science, (), . doi:doi.org/10.17660/eJHS.2021/86.1.4

Fine roots of apple rootstocks ‘M26’ and ‘Bittenfelder Sämling’ showing symptoms of apple replant disease (ARD) were histologically examined to clarify the relationships of microbial endophytes to tissue and cell damages. The plants were either grown on ARD-infested fields at three different locatio…